Structure-function mapping of BbCRASP-1, the key complement factor H and FHL-1 binding protein of Borrelia burgdorferi

Int J Med Microbiol. 2006 May:296 Suppl 40:177-84. doi: 10.1016/j.ijmm.2006.01.011. Epub 2006 Mar 10.

Abstract

Borrelia burgdorferi, a spirochaete transmitted to human hosts during feeding of infected Ixodes ticks, is the causative agent of Lyme disease, the most frequent vector-borne disease in Eurasia and North America. Sporadically Lyme disease develops into a chronic, multisystemic disorder. Serum-resistant B. burgdorferi strains bind complement factor H (FH) and FH-like protein 1 (FHL-1) on the spirochaete surface. This binding is dependent on the expression of proteins termed complement-regulator acquiring surface proteins (CRASPs). The atomic structure of BbCRASP-1, the key FHL-1/FH-binding protein of B. burgdorferi, has recently been determined. Our analysis indicates that its protein topology apparently evolved to provide a high affinity interaction site for FH/FHL-1 and leads to an atomic-level hypothesis for the functioning of BbCRASP-1. This work demonstrates that pathogens interact with complement regulators in ways that are distinct from the mechanisms used by the host and are thus obvious targets for drug design.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Binding Sites
  • Borrelia burgdorferi / chemistry*
  • Complement C3b Inactivator Proteins
  • Complement Factor H / metabolism
  • Humans
  • Membrane Proteins / chemistry*
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Structure-Activity Relationship

Substances

  • Bacterial Proteins
  • CFHR1 protein, human
  • Complement C3b Inactivator Proteins
  • Membrane Proteins
  • complement regulator-acquiring surface proteins, Borrelia burgdorferi
  • Complement Factor H