Distinguishing individual lipid headgroup mobility and phase transitions in raft-forming lipid mixtures with 31P MAS NMR

Biophys J. 2006 Jun 1;90(11):4248-60. doi: 10.1529/biophysj.105.077289. Epub 2006 Mar 13.

Abstract

A model membrane system composed of egg sphingomyelin (SM), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and cholesterol was studied with static and magic angle spinning (31)P NMR spectroscopy. This model membrane system is of significant biological relevance since it is known to form lipid rafts. (31)P NMR under magic angle spinning conditions resolves the SM and DOPC headgroup resonances allowing for extraction of the (31)P NMR parameters for the individual lipid components. The isotropic chemical shift, chemical shift anisotropy, and asymmetry parameter can be extracted from the spinning side band manifold of the individual components that form liquid-ordered and liquid-disordered domains. The magnitude of the (31)P chemical shift anisotropy and the line width is used to determine headgroup mobility and monitor the gel-to-gel and gel-to-liquid crystalline phase transitions of SM as a function of temperature in these mixtures. Spin-spin relaxation measurements are in agreement with the line width results, reflecting mobility differences and some heterogeneities. It will be shown that the presence of DOPC and/or cholesterol greatly impacts the headgroup mobility of SM both above and below the liquid crystalline phase transition temperature, whereas DOPC displays only minor variations in these lipid mixtures.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cholesterol / chemistry*
  • Lipid Bilayers / chemistry*
  • Membrane Fluidity*
  • Membrane Microdomains / chemistry*
  • Nuclear Magnetic Resonance, Biomolecular
  • Phase Transition
  • Phosphatidylcholines / chemistry*
  • Sphingomyelins / chemistry*
  • Spin Labels

Substances

  • Lipid Bilayers
  • Phosphatidylcholines
  • Sphingomyelins
  • Spin Labels
  • Cholesterol
  • 1,2-oleoylphosphatidylcholine