Abstract
We sequenced exon 1 of the UDP-glucuronosyltransferase (UGT) 1A7 gene from 52 Japanese cancer patients. Four single nucleotide polymorphisms (SNPs) were found. Three of them caused UGT1A7*2 and UGT1A7*3. A novel SNP (98973G>C) causing amino acid substitution (Ser141Cys) was found. The sequence is as follows: SNP, 050824FujitaK002; Gene Name, UGT1A7; Accession Number, AF297093; Length, 25 bases; 5'-TAAAGGAGAGTTG/CTTTTGATGCAGT-3'. One out of 52 cancer patients was heterozygous for the variant allele, resulting in the allele frequency of 0.96%. The patient did not possess UGT1A7*2 or UGT1A7*3.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Substitution
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Antineoplastic Agents, Phytogenic / pharmacokinetics
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Antineoplastic Agents, Phytogenic / therapeutic use
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Area Under Curve
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Camptothecin / analogs & derivatives
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Camptothecin / pharmacokinetics
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Camptothecin / therapeutic use
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DNA / genetics
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Exons / genetics
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Female
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Gene Frequency
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Glucuronosyltransferase / genetics*
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Humans
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Irinotecan
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Japan / epidemiology
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Male
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Molecular Sequence Data
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Neoplasms / enzymology
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Polymorphism, Single Nucleotide / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Antineoplastic Agents, Phytogenic
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Irinotecan
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DNA
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Glucuronosyltransferase
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UDP-glucuronosyltransferase, UGT1A7
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Camptothecin