Abstract
Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and D) were predicted based on sequence homology with the mycobacterial genes and confirmed experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about 10% of the wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene product, most likely the mca gene product (amidase).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylesterase / genetics
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Acetylesterase / metabolism
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Acetyltransferases / genetics*
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Acetyltransferases / metabolism
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Culture Media
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Cysteine
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Disaccharides / biosynthesis*
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Disaccharides / chemistry
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Gene Expression Regulation, Bacterial
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Glycopeptides
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Informatics
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Inositol
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Mutation
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Pyrazoles / chemistry
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Streptomyces coelicolor / enzymology
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Streptomyces coelicolor / genetics*
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Streptomyces coelicolor / growth & development
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Streptomyces coelicolor / metabolism*
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Sulfhydryl Compounds / chemistry
Substances
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Bacterial Proteins
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Culture Media
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Disaccharides
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Glycopeptides
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Pyrazoles
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Sulfhydryl Compounds
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mycothiol
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Inositol
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Acetyltransferases
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acetyl-CoA 1-D-myo-inosityl 2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside acetyltransferase
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Acetylesterase
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Cysteine