Suppression of tumorigenicity in Wilms tumor by the p15.5-p14 region of chromosome 11

Science. 1991 Oct 11;254(5029):293-5. doi: 10.1126/science.254.5029.293.

Abstract

Wilms tumor has been associated with genomic alterations at both the 11p13 and 11p15 regions. To differentiate between the involvement of these two loci, a chromosome 11 was constructed that had one or the other region deleted, and this chromosome was introduced into the tumorigenic Wilms tumor cell line G401. When assayed for tumor-forming activity in nude mice, the 11p13-deleted, but not the 11p15.5-p14.1-deleted chromosome, retained its ability to suppress tumor formation. These results provide in vivo functional evidence for the existence of a second genetic locus (WT2) involved in suppressing the tumorigenic phenotype of Wilms tumor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Genes, Tumor Suppressor / genetics*
  • Genes, Wilms Tumor* / genetics
  • Humans
  • Karyotyping
  • Kidney Neoplasms / genetics*
  • Mice
  • Mice, Nude
  • Wilms Tumor / genetics*