Abstract
It is known that Lassa virus Z protein is sufficient for the release of virus-like particles (VLPs) and that it has two L domains, PTAP and PPPY, in its C terminus. However, little is known about the cellular factor for Lassa virus budding. We examined which cellular factors are used in Lassa virus Z budding. We demonstrated that Lassa Z protein efficiently produces VLPs and uses cellular factors, Vps4A, Vps4B, and Tsg101, in budding, suggesting that Lassa virus budding uses the multivesicular body pathway functionally. Our data may provide a clue to develop an effective antiviral strategy for Lassa virus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Adenosine Triphosphatases / physiology
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Animals
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COS Cells
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Chlorocebus aethiops
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DNA-Binding Proteins / physiology
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Endosomal Sorting Complexes Required for Transport
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Lassa virus / physiology*
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Nedd4 Ubiquitin Protein Ligases
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RNA, Small Interfering / pharmacology
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Repressor Proteins / physiology
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Transcription Factors / physiology
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Transfection
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Ubiquitin-Protein Ligases / physiology
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Vacuolar Proton-Translocating ATPases
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Vesicular Transport Proteins
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Virion / physiology
Substances
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DNA-Binding Proteins
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Endosomal Sorting Complexes Required for Transport
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RNA, Small Interfering
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Repressor Proteins
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Transcription Factors
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Tsg101 protein
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Vesicular Transport Proteins
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Nedd4 Ubiquitin Protein Ligases
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Ubiquitin-Protein Ligases
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Adenosine Triphosphatases
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Vacuolar Proton-Translocating ATPases
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ATPases Associated with Diverse Cellular Activities
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VPS4A protein, human
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VPS4B protein, human