Abstract
The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA / genetics
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Gene Expression
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Proteoglycans / genetics*
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Proteoglycans / immunology
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RNA, Messenger / genetics
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Rats
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Receptors, Cell Surface / classification
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / immunology
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Receptors, Transforming Growth Factor beta
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Transfection
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Transforming Growth Factor beta / metabolism*
Substances
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Membrane Glycoproteins
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Membrane Proteins
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Proteoglycans
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Transforming Growth Factor beta
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Transforming Growth Factor beta
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betaglycan
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DNA
Associated data
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GENBANK/M80784
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GENBANK/S56945
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GENBANK/S60911
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GENBANK/S60914
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GENBANK/S60917
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GENBANK/S60919
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GENBANK/S60920
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GENBANK/S60969
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GENBANK/S61522
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GENBANK/S61524
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GENBANK/S65574