Abstract
In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Blotting, Western
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Drosophila / genetics
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Gene Expression Regulation / genetics
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HeLa Cells
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics*
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Simian virus 40 / genetics
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Sp1 Transcription Factor / genetics
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TATA Box / genetics*
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Transcription Factor TFIID
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Transcription Factors / antagonists & inhibitors
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic / genetics*
Substances
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Macromolecular Substances
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Recombinant Proteins
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Sp1 Transcription Factor
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Transcription Factor TFIID
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Transcription Factors