[Inhibitory effect of curcumin on angiogenesis induced by brain derived neurotrophic factor from multiple myeloma cells]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Feb;14(1):70-4.
[Article in Chinese]

Abstract

In order to explore the probability of curcumin treating multiple myeloma (MM) via the inhibition of angiogenesis, the expressions of brain derived neurotrophic factor (BDNF) and its specific receptor in human MM cells and endothelial cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The angiogenic activity was evaluated by endothelial cell migration assay and tubule formation assay in vitro. The results showed that exogenous BDNF significantly induced endothelial cell tubule formation and endothelial cell migration, these two effects were inhibited by curcumin. Furthermore, BDNF was detected in the MM cell and TrkB was detected in the endothelial cell and curcumin depressed the mRNA expression of BDNF and TrkB in the dose- and time-dependent manners. It is concluded that BDNF is a novel angiogenesis protein. Curcumin interrupts the interaction between multiple myeloma cells and endothelial cells by reducing TrkB expression in endothelial cells and inhibiting BDNF production in multiple myeloma cells, eventually, resulting in inhibition of angiogenesis. This is probably one part of the mechanism of the curcumin treating MM via the inhibition of angiogenesis.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / pharmacology*
  • Antineoplastic Agents / pharmacology
  • Brain-Derived Neurotrophic Factor / antagonists & inhibitors*
  • Brain-Derived Neurotrophic Factor / biosynthesis
  • Brain-Derived Neurotrophic Factor / genetics
  • Cell Line, Tumor
  • Curcumin / pharmacology*
  • Endothelial Cells / cytology
  • Humans
  • Multiple Myeloma / blood supply*
  • Multiple Myeloma / pathology
  • Neovascularization, Pathologic / prevention & control*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics

Substances

  • Angiogenesis Inhibitors
  • Antineoplastic Agents
  • Brain-Derived Neurotrophic Factor
  • RNA, Messenger
  • Curcumin