Abstract
In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay. The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with baculovirus itself possibly owing to increased stability of baculovirus by pegylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Baculoviridae / chemistry
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Baculoviridae / genetics*
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Cell Line
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Cell Line, Tumor
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Cells, Cultured
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Genetic Vectors / chemistry
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Genetic Vectors / genetics*
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Genetic Vectors / pharmacokinetics
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Green Fluorescent Proteins / pharmacokinetics
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Humans
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Mice
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Microscopy, Fluorescence
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Polyethylene Glycols / chemistry*
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Polyethylene Glycols / pharmacology
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / pharmacokinetics
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Tissue Distribution / drug effects
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Transfection / methods*
Substances
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Polyethylene Glycols