Abstract
We have measured mu and delta opioid receptor sites on intact SK-N-SH and NG108-15 neuroblastoma cells, respectively, in culture. Use of 125I-beta-endorphin (beta E) as a tracer, together with beta E(6-31) to block high-affinity non-opioid binding in both cell lines, permitted the measurement of cell surface mu and delta opioid receptor sites. Labeling was at delta sites in NG108-15 cells and predominantly at mu sites in SK-N-SH cells. Pretreatment with the mu and delta agonist, DADLE, caused a rapid loss of cell surface delta receptor sites in NG108-15 cells, but failed to reduce significantly mu receptor density in SK-N-SH cells.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Analgesics / pharmacology
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Animals
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Binding Sites / drug effects
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalin, D-Penicillamine (2,5)-
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Enkephalin, Leucine-2-Alanine / pharmacology
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Enkephalins / pharmacology
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Humans
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Mice
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Neuroblastoma / metabolism*
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Receptors, Opioid / drug effects*
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Receptors, Opioid / metabolism
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Receptors, Opioid, delta
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Receptors, Opioid, mu
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Tumor Cells, Cultured / metabolism
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beta-Endorphin / metabolism
Substances
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Analgesics
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Enkephalins
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Receptors, Opioid
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Receptors, Opioid, delta
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Receptors, Opioid, mu
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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beta-Endorphin
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Enkephalin, Leucine-2-Alanine
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Enkephalin, D-Penicillamine (2,5)-