The interplay of the concepts of complementarity and interference in the time-energy domain are studied. In particular, we theoretically investigate the fluorescence light from a J = 1/2 to J= 1/2 transition that is driven by a monochromatic laser field. We find that the spectrum of resonance fluorescence exhibits a signature of vacuum-mediated interference effects, whereas the total intensity is not affected by interference. We demonstrate that this result is a consequence of the principle of complementarity, applied to time and energy. Since the considered level scheme can be found, e.g., in (198)Hg(+) ions, our model system turns out to be an ideal candidate to provide evidence for as yet experimentally unconfirmed vacuum-induced atomic coherences.