Abstract
To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Genetic Vectors
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Humans
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Laminin / biosynthesis*
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Laminin / genetics
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Pichia / genetics
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Pichia / metabolism*
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Plasmids / genetics
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification*
Substances
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LAMA4 protein, human
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Laminin
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Recombinant Fusion Proteins