Abstract
We have cloned and characterized cDNAs coding for a receptor-class phosphotyrosine phosphatase gene from Drosophila melanogaster. The gene maps to the polytene chromosome bands 99A7-8. The cDNA clones code for a polypeptide of 1301 amino acids with a predicted molecular mass of 145 kDa. The extracellular domain includes two fibronectin-type III-like domains. The cytoplasmic region contains two tandemly repeated phosphotyrosine phosphatase-like domains. Residues shown crucial for catalytic activity are absent in the second domain. This Drosophila receptor-class phosphotyrosine phosphatase polypeptide is expressed on axons of the embryonic central nervous system.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies
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Axons / enzymology*
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Base Sequence
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Chromosome Banding
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Cloning, Molecular
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DNA Probes
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Drosophila melanogaster / embryology
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Drosophila melanogaster / enzymology
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Drosophila melanogaster / genetics*
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Fibronectins / genetics
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Humans
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Molecular Sequence Data
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Nervous System / enzymology
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction / methods
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Protein Tyrosine Phosphatases / genetics*
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Protein Tyrosine Phosphatases / isolation & purification
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Receptors, Cell Surface / genetics*
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Repetitive Sequences, Nucleic Acid
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Restriction Mapping
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Sequence Homology, Nucleic Acid
Substances
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Antibodies
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DNA Probes
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Fibronectins
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Oligodeoxyribonucleotides
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Receptors, Cell Surface
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Protein Tyrosine Phosphatases
Associated data
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GENBANK/M59745
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GENBANK/M59746
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GENBANK/M59747
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GENBANK/M59748
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GENBANK/M80723
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GENBANK/M80724
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GENBANK/M81664
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GENBANK/M81665
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GENBANK/M81666
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GENBANK/M81795