Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216

Protein Expr Purif. 2006 Jun;47(2):393-7. doi: 10.1016/j.pep.2006.02.007. Epub 2006 Mar 10.

Abstract

We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively. The lipase genes with 6 x His tag sequence were cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host Pichia pastoris KM71, respectively. The recombinants were induced by methanol to secrete active lipases into cultural medium. The recombinant lipases were also purified and characterized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / isolation & purification*
  • Cloning, Molecular
  • Gene Expression
  • Lipase / biosynthesis
  • Lipase / isolation & purification*
  • Pichia
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification*
  • Yarrowia / enzymology*
  • Yarrowia / genetics

Substances

  • Bacterial Proteins
  • Recombinant Proteins
  • Lipase