A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus

J Vet Med B Infect Dis Vet Public Health. 2006 Mar;53(2):59-61. doi: 10.1111/j.1439-0450.2006.00915.x.

Abstract

A slide latex agglutination test (LAT) was developed and evaluated to detect serum antibodies against porcine parvovirus. Porcine parvovirus antigen was obtained by 10% PEG-6000 and 0.5 mol/l sodium chloride precipitation, and inactivated by 0.1% methanal. Two per cent suspensions of latex particles (0.5-0.8 microm) were coated by adding an equal volume of porcine parvovirus antigen at 0.34 microg/ml. Repeatability of latex agglutination test was evaluated with a panel of 100 sera using the same and different antigen lots. A good agreement between LAT and haemagglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Latex Fixation Tests / methods
  • Latex Fixation Tests / veterinary*
  • Parvoviridae Infections / diagnosis
  • Parvoviridae Infections / veterinary*
  • Parvovirus, Porcine / immunology*
  • Sensitivity and Specificity
  • Swine
  • Swine Diseases / diagnosis*
  • Time Factors

Substances

  • Antibodies, Viral