Abstract
A novel alkalitolerant, sulphate-reducing bacterium (strain RT2T) was isolated from alkaline district heating water. Strain RT2T was a motile vibrio (0.5-0.8 microm wide and 1.4-1.9 microm long) and grew at pH 6.9-9.9 (optimum at pH 9.0-9.4) and at 16-47 degrees C (optimum at 43 degrees C). The genomic DNA G+C content was 64.7 mol%. A limited number of compounds were used as electron donors with sulphate as electron acceptor, including lactate, pyruvate, formate and hydrogen/acetate. Sulphite and thiosulphate also served as electron acceptors. Based on physiological and genotypic properties, the isolate was considered to represent a novel species of the genus Desulfovibrio, for which the name Desulfovibrio alkalitolerans sp. nov. is proposed. The type strain is RT2T (=DSM 16529T=JCM 12612T). The strain is the first alkali-tolerant member of the genus Desulfovibrio to be described.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetic Acid / metabolism
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Alkalies / pharmacology*
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Anti-Bacterial Agents / pharmacology
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Base Composition
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Denmark
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Desulfovibrio / classification*
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Desulfovibrio / isolation & purification*
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Desulfovibrio / metabolism
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Desulfovibrio / physiology
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Formates / metabolism
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Genes, rRNA
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Hydrogen / metabolism
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Hydrogen-Ion Concentration
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Lactic Acid / metabolism
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Molecular Sequence Data
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Movement
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Oxidation-Reduction
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Phylogeny
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Pyruvic Acid / metabolism
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Sulfates / metabolism*
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Sulfites / metabolism
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Temperature
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Water Microbiology*
Substances
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Alkalies
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Anti-Bacterial Agents
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DNA, Bacterial
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DNA, Ribosomal
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Formates
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Sulfates
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Sulfites
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formic acid
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Lactic Acid
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Hydrogen
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Pyruvic Acid
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Acetic Acid
Associated data
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GENBANK/AY649785
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GENBANK/AY649786
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GENBANK/AY744465