Increased number of anaerobic bacteria in the infected root canal in type 2 diabetic rats

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 May;101(5):681-6. doi: 10.1016/j.tripleo.2005.06.007. Epub 2005 Oct 12.

Abstract

Objective: The purpose of this study was to investigate the relationship between type 2 diabetes mellitus and anaerobic bacteria detected in infected root canals.

Study design: Normal Wistar rats (control) received a standard laboratory diet with water (group A), and GK rats (type 2 diabetes mellitus rats) a normal laboratory diet with water (group B) or a 30% sucrose solution (group C). Chemotaxis assay was conducted on polymorphonuclear leukocytes from the 3 groups, and the numbers of anaerobic bacteria in infected root canals were determined.

Results: In the chemotaxis assay on the polymorphonuclear leukocytes, the chemotactic response of cells in group C was lower than that for groups A and B (P < .01). As to bacteria detected in the root canal, obligate anaerobic bacteria which stained gram negative, were significantly more numerous in group C (P < .01) than in groups A and B.

Conclusion: The metabolic condition produced by type 2 diabetes mellitus in rats might lower the general host resistance against bacterial infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria, Anaerobic / isolation & purification
  • Bacterial Infections / complications*
  • Bacterial Infections / immunology
  • Bacterial Infections / microbiology
  • Chemotaxis, Leukocyte
  • Dental Pulp Cavity / microbiology*
  • Dental Pulp Necrosis / complications
  • Dental Pulp Necrosis / immunology
  • Dental Pulp Necrosis / microbiology*
  • Diabetes Mellitus, Type 2 / complications*
  • Diabetes Mellitus, Type 2 / immunology
  • Disease Models, Animal
  • Gram-Negative Bacteria / isolation & purification
  • Neutrophils / immunology
  • Rats
  • Rats, Inbred Strains
  • Rats, Wistar