Full-field swept-source phase microscopy

Opt Lett. 2006 May 15;31(10):1462-4. doi: 10.1364/ol.31.001462.

Abstract

We present a full-field phase microscopy technique for quantitative nanoscale surface profiling of samples in reflection. This technique utilizes swept-source optical coherence tomography in a full-field common path interferometer for phase-stable cross-sectional acquisition without scanning. Subwavelength variations in surface sample features are measured without interference from spurious reflections by processing the interferometric phase at a selected depth plane, providing a 1.3 nm stability for high signal-to-noise ratio surface features. Nanoscale imaging was demonstrated by measuring the location of receptor sites on a DNA assay biochip and the surface topography of erythrocytes in a blood smear.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cells, Cultured
  • Equipment Design
  • Equipment Failure Analysis
  • Erythrocytes / ultrastructure*
  • Humans
  • Image Enhancement / instrumentation
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Microscopy, Phase-Contrast / instrumentation*
  • Microscopy, Phase-Contrast / methods
  • Nanotechnology / instrumentation*
  • Nanotechnology / methods
  • Tomography, Optical Coherence / instrumentation*
  • Tomography, Optical Coherence / methods