Cutting edge: constitutive B cell receptor signaling is critical for basal growth of B lymphoma

J Immunol. 2006 May 15;176(10):5715-9. doi: 10.4049/jimmunol.176.10.5715.

Abstract

B lymphomas account for the majority of the lymphoma cases. BCR expression appears to be important for B lymphoma because most oncogenes are translocated to nonrearranged Ig loci and because all of the variants that arise in anti-idiotypic Ab-treated lymphoma patients remain BCR positive. Based on this and the fact that BCR is required for mature B cell survival, we tested the requirement for continued expression of BCR for the growth and survival of B lymphoma cells. Using Igalpha or Igbeta-specific small interfering RNA (siRNA) to inhibit BCR expression, we demonstrate for the first time that constitutive signaling by BCR is critical for survival and proliferation of both murine and human B lymphoma cells. The BCR signals in lymphoma appear to be mediated by Syk, as it is constitutively active in a variety of B lymphoma cells. Blocking Syk activity by selective inhibitors suppresses growth of several murine and human B lymphomas.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Proliferation* / drug effects
  • Growth Inhibitors / pharmacology
  • Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Lymphoma, B-Cell / immunology
  • Lymphoma, B-Cell / metabolism*
  • Lymphoma, B-Cell / pathology*
  • Male
  • Mice
  • Protein Kinase Inhibitors / pharmacology
  • Protein-Tyrosine Kinases / antagonists & inhibitors
  • Protein-Tyrosine Kinases / metabolism
  • Receptors, Antigen, B-Cell / physiology*
  • Signal Transduction / immunology*
  • Syk Kinase

Substances

  • Growth Inhibitors
  • Intracellular Signaling Peptides and Proteins
  • Protein Kinase Inhibitors
  • Receptors, Antigen, B-Cell
  • Protein-Tyrosine Kinases
  • SYK protein, human
  • Syk Kinase
  • Syk protein, mouse