Abstract
The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Typing Techniques
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Base Sequence
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DNA Primers / genetics
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DNA Probes / genetics
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Genes, Bacterial
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Genotype
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Humans
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Staphylococcus aureus / classification*
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / isolation & purification
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Staphylococcus aureus / pathogenicity
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Trans-Activators / genetics*
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Virulence / genetics
Substances
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Agr protein, Staphylococcus aureus
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Bacterial Proteins
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DNA Primers
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DNA Probes
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DNA, Bacterial
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Trans-Activators