Abstract
Background information:
Exosomes are small membrane vesicles secreted by several cell types during exocytic fusion of multivesicular bodies with the plasma membrane. Exosomes from tumour cells can transfer antigens from cell to cell, a property favouring antigen-specific immune responses in vitro and in vivo, and are thus an interesting putative therapeutic tool in human cancers. Exosomes have been well studied in EBV (Epstein-Barr virus)-transformed human B-cell lines; however, biological stimuli regulating exosome secretion quantitatively and/or qualitatively still remain poorly defined.
Results:
We analysed the effect of the BCR stimulation on exosome release in the human follicular lymphoma B-cell line DOHH2. We found that BCR (B-cell receptor) triggering of DOHH2 cells induced the polarization of CD63(+) MHC class II compartments. Moreover, BCR stimulation increased the release of exosome-associated proteins in the extracellular space. Finally, we found that the BCR was expressed at the surface of exosomes, and could target a bound anti-human IgG to these vesicles.
Conclusions:
BCR can modulate the protein content of exosomes upon stimulation, and can target its bound antigen to these vesicles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-ribosyl Cyclase 1 / analysis
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Antigens / metabolism*
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Antigens, CD / analysis
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Antigens, CD / metabolism
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Antigens, CD20 / analysis
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B7-2 Antigen / analysis
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Cell Line, Tumor
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Cell Membrane / chemistry
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Cell Polarity / immunology
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Exocytosis / immunology*
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HLA-D Antigens / analysis
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HLA-D Antigens / metabolism
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Histocompatibility Antigens Class I / analysis
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Histocompatibility Antigens Class I / metabolism
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Humans
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Immunophenotyping
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Lymphocyte Activation / immunology
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Lymphoma, B-Cell / immunology
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Lymphoma, B-Cell / metabolism
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Lymphoma, B-Cell / pathology
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Lymphoma, Follicular / immunology
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Lymphoma, Follicular / metabolism
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Lymphoma, Follicular / pathology
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Membrane Proteins / analysis
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Membrane Proteins / metabolism
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Microscopy, Confocal
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Phosphorylation
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Platelet Membrane Glycoproteins / analysis
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Platelet Membrane Glycoproteins / metabolism
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Protein Transport / immunology
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Receptors, Antigen, B-Cell / analysis
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Receptors, Antigen, B-Cell / metabolism*
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Receptors, Transferrin / analysis
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Receptors, Transferrin / metabolism
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Tetraspanin 30
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Transport Vesicles / immunology*
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Transport Vesicles / metabolism
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Transport Vesicles / ultrastructure
Substances
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Antigens
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Antigens, CD
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Antigens, CD20
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B7-2 Antigen
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CD63 protein, human
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HLA-D Antigens
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Histocompatibility Antigens Class I
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Membrane Proteins
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Platelet Membrane Glycoproteins
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Receptors, Antigen, B-Cell
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Receptors, Transferrin
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Tetraspanin 30
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flotillins
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ADP-ribosyl Cyclase 1