Abstract
Ochnaflavone is a medicinal herbal product isolated from Lonicera japonica that inhibits cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with IC50 values of 0.6 microM. Western blotting probed with specific anti-COX-2 antibodies showed that the decrease in quantity of the PGD2 product was accompanied by a decrease in the COX-2 protein level. In addition, this compound consistently inhibited the production of leukotriene C4 (LTC4) in a dose dependent manner, with an IC50 value of 6.56 microM. These results demonstrate that ochnaflavone has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity. Furthermore, this compound strongly inhibited degranulation reaction in a dose dependent manner, with an IC50 value of 3.01 microM. Therefore, this compound might provide a basis for novel anti-inflammatory drugs.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Arachidonate 5-Lipoxygenase / metabolism
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / physiology
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Cell Degranulation
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Cyclooxygenase 2 / metabolism
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Cyclooxygenase 2 Inhibitors / isolation & purification
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Cyclooxygenase 2 Inhibitors / pharmacology*
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Dose-Response Relationship, Drug
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Flavonoids / isolation & purification
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Flavonoids / pharmacology*
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Leukotriene C4 / metabolism
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Lipoxygenase Inhibitors* / isolation & purification
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Lipoxygenase Inhibitors* / pharmacology*
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Lonicera* / chemistry
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Male
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Mast Cells / drug effects*
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Mast Cells / physiology
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Mice
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Mice, Inbred BALB C
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Prostaglandin D2 / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Cyclooxygenase 2 Inhibitors
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Flavonoids
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Lipoxygenase Inhibitors
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Leukotriene C4
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ochnaflavone
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Arachidonate 5-Lipoxygenase
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Cyclooxygenase 2
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Prostaglandin D2