The HIV-1 matrix protein p17 can be efficiently delivered by intranasal route in mice using the TLR 2/6 agonist MALP-2 as mucosal adjuvant

Pablo D Becker; Simona Fiorentini; Claudia Link; Giorgio Tosti; Thomas Ebensen; Arnaldo Caruso; Carlos A Guzmán

doi:10.1016/j.vaccine.2005.11.008

The HIV-1 matrix protein p17 can be efficiently delivered by intranasal route in mice using the TLR 2/6 agonist MALP-2 as mucosal adjuvant

Vaccine. 2006 Jun 19;24(25):5269-76. doi: 10.1016/j.vaccine.2005.11.008. Epub 2005 Nov 21.

Authors

Pablo D Becker¹, Simona Fiorentini, Claudia Link, Giorgio Tosti, Thomas Ebensen, Arnaldo Caruso, Carlos A Guzmán

Affiliation

¹ Department of Vaccinology, GBF-German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.

PMID: 16713032
DOI: 10.1016/j.vaccine.2005.11.008

Abstract

The HIV-1 matrix protein p17 is a structural protein essential in the life cycle of HIV, by acting as a virokine/immunomodulator that supports viral replication and spreading. The presence of p17-specific antibodies and CTL responses correlates with slower progression to AIDS. Intranasal vaccination with p17 and the TLR2/6 agonist MALP-2 stimulates strong humoral and cellular immune responses at systemic and mucosal levels. The antibodies blocked p17 binding to its receptor, which is a critical step for the exertion of its virokine activity. Our results suggest that p17 and MALP-2 are attractive candidates for incorporation in mucosal vaccines against HIV/AIDS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AIDS Vaccines / administration & dosage*
AIDS Vaccines / immunology
Adjuvants, Immunologic / administration & dosage*
Administration, Intranasal
Animals
Female
Gene Products, gag / administration & dosage*
Gene Products, gag / chemical synthesis
Gene Products, gag / chemistry
Gene Products, gag / immunology
HIV Antibodies / blood
HIV Antibodies / metabolism
HIV Antigens / administration & dosage*
HIV Antigens / chemistry
HIV Antigens / immunology
HIV Infections / immunology
HIV Infections / prevention & control
HIV-1 / immunology*
Humans
Immunity, Mucosal
Immunoglobulin A, Secretory / metabolism
Immunoglobulin G / blood
Lipopeptides
Mice
Mice, Inbred BALB C
Oligopeptides / administration & dosage*
Oligopeptides / immunology
Th1 Cells / immunology
Th2 Cells / immunology
Toll-Like Receptor 2
Viral Proteins / administration & dosage*
Viral Proteins / chemical synthesis
Viral Proteins / chemistry
Viral Proteins / immunology
gag Gene Products, Human Immunodeficiency Virus

Substances

AIDS Vaccines
Adjuvants, Immunologic
Gene Products, gag
HIV Antibodies
HIV Antigens
Immunoglobulin A, Secretory
Immunoglobulin G
Lipopeptides
Oligopeptides
Toll-Like Receptor 2
Viral Proteins
gag Gene Products, Human Immunodeficiency Virus
p17 protein, Human Immunodeficiency Virus Type 1
macrophage stimulatory lipopeptide 2