Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast

M B Singh; T Hough; P Theerakulpisut; A Avjioglu; S Davies; P M Smith; P Taylor; R J Simpson; L D Ward; J McCluskey

doi:10.1073/pnas.88.4.1384

Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast

Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1384-8. doi: 10.1073/pnas.88.4.1384.

Authors

M B Singh¹, T Hough, P Theerakulpisut, A Avjioglu, S Davies, P M Smith, P Taylor, R J Simpson, L D Ward, J McCluskey, et al.

Affiliation

¹ School of Botany, University of Melbourne, Parkville, Victoria, Australia.

Abstract

We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allergens*
Amino Acid Sequence
Antibodies, Monoclonal
Antigens, Plant
Base Sequence
Cloning, Molecular
DNA / genetics*
DNA / isolation & purification
Gene Library
Lolium / genetics*
Lolium / immunology
Molecular Sequence Data
Plant Proteins / genetics*
Plant Proteins / immunology
Pollen / analysis*
Pollen / immunology*
Pollen / ultrastructure
Poly A / genetics
Poly A / isolation & purification
Protein Conformation
RNA, Messenger / genetics
RNA, Messenger / isolation & purification
Restriction Mapping

Substances

Allergens
Antibodies, Monoclonal
Antigens, Plant
Lol p I protein, Lolium perenne
Plant Proteins
RNA, Messenger
Poly A
DNA

Associated data

GENBANK/M59163