[Application of four ELISA techniques (two for IgM and two for IgG) for serological diagnosis of an outbreak of Q fever]

Enferm Infecc Microbiol Clin. 2006 Mar;24(3):178-81. doi: 10.1157/13086551.
[Article in Spanish]

Abstract

Objective: To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of Q fever.

Methods: Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method.

Results: The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%.

Conclusions: Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / blood*
  • Coxiella burnetii / immunology*
  • Disease Outbreaks
  • Enzyme-Linked Immunosorbent Assay* / methods
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Q Fever / diagnosis*
  • Q Fever / epidemiology
  • Sensitivity and Specificity

Substances

  • Antibodies, Bacterial
  • Immunoglobulin G
  • Immunoglobulin M