Abstract
A Streptomyces sp. isolated from riverbank soil in Manitoba, Canada, was found to contain two cancer cell growth inhibitories: diazaanthraquinone 1 and 3-(hydroxyacetyl)indole (8). The structures were determined by interpretation of data from HRMS, UV, and high-field (400 MHz) NMR experiments. The red-colored diazaanthraquinone 1 and 3-(hydroxyacetyl)indole (8) were found to inhibit (0.1-3 microg/mL) growth of a minipanel of human cancer cell lines and P388 lymphocytic leukemia cells. Diazaanthraquinone 1 was also found to inhibit growth of the bacteria Streptococcus pneumoniae and Neisseria gonorrheae. However, three companion constituents, cyclo-Pro-Leu (5), cyclo-Pro-Phe (6), and cyclo-Pro-Val (7), did not inhibit cancer cell growth.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Aza Compounds / chemistry
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Aza Compounds / isolation & purification*
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Aza Compounds / pharmacology
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Dipeptides / isolation & purification
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Drug Screening Assays, Antitumor
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Humans
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Indoles / chemistry
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Indoles / isolation & purification*
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Indoles / pharmacology
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Leukemia P388
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Manitoba
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Mice
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Molecular Structure
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Peptides, Cyclic / isolation & purification
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Quinones / chemistry
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Quinones / isolation & purification*
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Quinones / pharmacology
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Streptococcus / drug effects
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Streptomyces / chemistry*
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Streptomyces / isolation & purification
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Tumor Cells, Cultured
Substances
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3-(hydroxyacetyl)indole
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Anti-Bacterial Agents
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Aza Compounds
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Dipeptides
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Indoles
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Peptides, Cyclic
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Quinones
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Sch 538415
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cyclo(prolyl-leucyl)
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cyclo(prolyl-valyl)