Abstract
A novel, structurally distinct POU-domain protein has been identified that inhibits activation by another positive POU-domain regulator of neuron-specific transcription units. Two Drosophila POU-domain proteins, I-POU and Cf1-a, are coexpressed in overlapping subsets of neurons during development. Because I-POU lacks two basic residues in the N terminus of its homeodomain, it cannot bind DNA, but it does form a stable heterodimeric complex with Cf1-a, preventing Cf1-a from binding to DNA recognition elements and from transactivating the dopa-decarboxylase gene. The inhibition by I-POU provides a potential strategy by which the activation of genes in development is controlled by a homeodomain-containing protein that does not bind DNA.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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Dopa Decarboxylase / genetics
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Drosophila / genetics
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Drosophila / growth & development
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Drosophila Proteins*
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Gene Expression Regulation*
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Genes, Homeobox
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Neurons / physiology*
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POU Domain Factors
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RNA, Messenger / genetics
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Sequence Homology, Nucleic Acid
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Transcription Factors*
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Transcription, Genetic
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Transcriptional Activation
Substances
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DNA-Binding Proteins
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Drosophila Proteins
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Nerve Tissue Proteins
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POU Domain Factors
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RNA, Messenger
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Transcription Factors
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acj6 protein, Drosophila
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Dopa Decarboxylase
Associated data
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GENBANK/X57450
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GENBANK/X57451
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GENBANK/X57452
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GENBANK/X57453
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GENBANK/X57454
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GENBANK/X57455
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GENBANK/X57688
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GENBANK/X57689
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GENBANK/X58435
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GENBANK/X58436