Size calculation of restriction enzyme HaeIII-generated fragments detected by probe YNH24 by comparison of data from two laboratories: the generation of fragment-size frequencies

Forensic Sci Int. 1991 Jan-Feb;49(1):21-31. doi: 10.1016/0379-0738(91)90168-i.

Abstract

Restriction fragment-length polymorphism of locus D2S44 detected by the highly polymorphic probe YNH24 and restriction endonuclease HaeIII can be used to improve parentage testing when representative fragment-size frequencies can be obtained. By joining the results of different laboratories, it is possible to set up a meaningful databank. Therefore, the same randomly chosen samples were tested for the HaeIII RFLP detected by probe YNH24 in Düsseldorf (DUS) and Amsterdam (AMS). The results of the different fragment-size calculations obtained by using internal markers and a computerized system (DUS-cad and AMS-cad), and by using external markers and manual calculations (DUS-man), were analyzed. Comparing these results, no statistically significant differences were seen. The results obtained with probe YNH24 and enzyme HaeIII in Düsseldorf and Amsterdam can be used to attain a sufficient number of samples to generate relevant fragment-size frequencies.

Publication types

  • Comparative Study

MeSH terms

  • DNA / chemistry*
  • DNA Probes*
  • Deoxyribonucleases, Type II Site-Specific*
  • Electrophoresis, Agar Gel
  • Humans
  • Nucleic Acid Hybridization
  • Paternity
  • Polymorphism, Restriction Fragment Length*
  • Reproducibility of Results

Substances

  • DNA Probes
  • DNA
  • Deoxyribonucleases, Type II Site-Specific
  • GGCC-specific type II deoxyribonucleases