Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt 6):559-61. doi: 10.1107/S1744309106016472. Epub 2006 May 31.

Abstract

In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 36.14, b = 38.96, c = 30.81 A, beta = 110.69 degrees .

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Crystallization
  • Nucleic Acid Conformation
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / chemistry*
  • RNA, Transfer, Ser / chemistry*
  • X-Ray Diffraction

Substances

  • Oligonucleotides
  • RNA, Transfer, Ser

Associated data

  • PDB/1EHZ
  • PDB/434D