Role of vitamin E-coated membrane in reducing advanced glycation end products in hemodialysis patients: a pilot study

Blood Purif. 2006;24(4):369-76. doi: 10.1159/000093678. Epub 2006 Jun 1.

Abstract

Introduction: Advanced glycation end products (AGEs) are markers of oxidative stress.

Aims: To assess if a vitamin-E-coated dialyzer affects plasma AGE levels and endothelial function in hemodialysis patients.

Methods: 16 patients were dialyzed with a synthetic modified cellulose membrane (SMC, n = 8) or a vitamin E-coated dialyzer (n = 8), respectively. At week 32 endothelial function was determined as brachial artery flow-mediated dilatation (FMD). Total AGEs, free pentosidine (FP), protein-bound pentosidine (BP) and autoantibodies against oxidized LDL (ox-LDL-autoantibodies) were assessed at baseline (T0) and at 16, 32, 40 and 42 weeks (T16, T32, T40 and T42).

Results: At T16 and T32 FP and BP were lower in vitamin E than in SMC (T 16: 88.7 +/- 8.96 vs. 124.2 +/- 11.90 pmol/ml plasma; p = 0.04, and 22.9 +/- 2.99 vs. 32.8 +/- 2.98 pmol/mg proteins; p = 0.04. T32: 78.7 +/- 8.54 vs. 123.7 +/- 10.15 pmol/ml plasma; p = 0.007, and 19.9 +/- 2.0 vs. 33.67 +/- 2.41 pmol/mg proteins; p = 0.001). In vitamin E, AGEs were lower at T32, T40 and T42 (946.7 +/- 80.91 vs. 1,351.2 +/- 179.33 AU/ml, p = 0.05; 986.9 +/- 59.63 vs. 1,509.9 +/- 154.17 AU/ml, p = 0.013; 890.3 +/- 73.70 vs. 1,453.9 +/- 153.16 AU/ml, p = 0.009). At T32 AGEs, ox-LDL autoantibodies and FMD were inversely correlated (R = -0.70 p = 0.007 and R = -0.59, p = 0.04, respectively).

Conclusions: Vit E-coated membrane reduces plasma AGEs levels and AGEs values are negatively correlated with FMD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Analysis of Variance
  • Antioxidants / pharmacology*
  • Arginine / analogs & derivatives
  • Arginine / blood
  • Brachial Artery / drug effects
  • Brachial Artery / physiopathology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiopathology
  • Female
  • Glycation End Products, Advanced / blood*
  • Homocysteine / drug effects
  • Humans
  • Linear Models
  • Lipoproteins, LDL / immunology
  • Lysine / analogs & derivatives
  • Lysine / blood
  • Male
  • Membranes, Artificial*
  • Middle Aged
  • Oxidative Stress / drug effects
  • Pilot Projects
  • Renal Dialysis*
  • Vasodilation / drug effects
  • Vasodilation / physiology
  • Vitamin E / pharmacology*

Substances

  • Antioxidants
  • Glycation End Products, Advanced
  • Lipoproteins, LDL
  • Membranes, Artificial
  • Homocysteine
  • Vitamin E
  • Arginine
  • pentosidine
  • Lysine