Abstract
Histone modifications are central to epigenetic regulation and must be reestablished with each round of DNA replication. Here we describe methods to localize these modifications within mammalian nuclei and to relate them to specific spatiotemporal patterns of DNA replication.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies / chemistry*
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Bromodeoxyuridine / pharmacology
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Cattle
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Cell Nucleus / metabolism
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DNA Methylation
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DNA Replication
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Ethanol / chemistry
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Fluorescent Dyes / pharmacology*
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Histones / chemistry*
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods*
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S Phase*
Substances
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Antibodies
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Fluorescent Dyes
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Histones
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Ethanol
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Bromodeoxyuridine