Recombinant adenovirus expressing GP5 and M fusion proteins of porcine reproductive and respiratory syndrome virus induce both humoral and cell-mediated immune responses in mice

Vet Immunol Immunopathol. 2006 Sep 15;113(1-2):169-80. doi: 10.1016/j.vetimm.2006.05.001. Epub 2006 Jun 13.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important contagious agents of swine in the world. PRRSV infection poses a challenge to current vaccination strategies. In this study, three replication-defective adenovirus recombinants were developed as potential vaccine against PRRSV in a mouse model. Three groups of BALB/c mice (24 mice per group) were inoculated subcutaneously twice at 2-week intervals with the recombinants expressing PRRSV GP5 (rAd-GP5), M (rAd-M), and M-GP5 fusion protein (rAd-M-GP5). Two additional groups were injected with wild-type adenovirus (wtAd) or PBS as control. The results showed that the mice inoculated with recombinant adenoviruses developed PRRSV-specific antibodies, cellular immune response by 2 weeks post second inoculation. However, only mice immunized with recombinant adenovirus rAd-M-GP5 developed significantly higher titers of neutralizing antibodies to PRRSV and produced stronger lymphocyte proliferation responses compared to mice immunized with rAd-M or rAd-GP5 alone. It was also found that mice immunized with rAd-M-GP5 were primed for significant higher levels of anti-PRRSV CTL responses than mice immunized with rAd-M. Mice receiving rAd-GP5 also mounted PRRSV-specific response, but levels were lower. It suggested that the recombinant adenovirus expressing M-GP5 fusion protein might be an attractive candidate vaccine to be tested for preventing PRRSV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Antibodies, Viral / blood
  • DNA, Viral / genetics
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Genetic Vectors
  • Mice
  • Mice, Inbred BALB C
  • NIH 3T3 Cells
  • Neutralization Tests / veterinary
  • Polymerase Chain Reaction / veterinary
  • Porcine Reproductive and Respiratory Syndrome / immunology*
  • Porcine Reproductive and Respiratory Syndrome / prevention & control
  • Porcine respiratory and reproductive syndrome virus / genetics
  • Porcine respiratory and reproductive syndrome virus / immunology*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Swine
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccination / methods
  • Vaccination / veterinary
  • Viral Envelope Proteins / biosynthesis
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*
  • Viral Matrix Proteins / biosynthesis
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / immunology*
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Recombinant Fusion Proteins
  • Viral Envelope Proteins
  • Viral Matrix Proteins
  • Viral Vaccines
  • glycoprotein 5, PRRSV