Analysis of L-myc gene polymorphism in patients with renal failure outcome to renal transplant

Transplant Proc. 2006 Jun;38(5):1267-9. doi: 10.1016/j.transproceed.2006.03.037.

Abstract

Background: Abnormalities of cell numbers and apoptosis have been observed in renal failure. As uncontrolled expression of c-myc is known to induce apoptosis, we thought that polymorphism in the other myc gene, L-myc gene, which is structually similar to c-myc and reported to be expressed in the kidney, may have a role in the induction of apoptosis and thus have role in chronic renal failure. The aim of this study was to investigate the relationship between the distribution of L-myc genotypes and renal failure.

Methods: In the present study we examined 101 chronic renal failure patients who had either live or cadaveric renal transplants and 105 healthy individuals, for L-myc gene polymorphism by polymerase chain reactions and restriction fragment length polymorphism techniques.

Results: Among our patient group, the distribution of the LL, LS, and SS genotypes was 24% (n=25), 71% (n=71), and 5% (n=5), respectively, versus 41% (n=43), 47% (n=49), and 12% (n=13) in our control group. The distribution of genotypes was significantly different between our patients and the control group (chi2=12.281; P=.002). The frequency of the S allele was significantly higher in the patient group (chi2=6.122; P=.013).

Conclusion: Our study showed that having an S allele in the L-myc gene may increase the risk of renal failure.

MeSH terms

  • Genes, myc*
  • Genotype
  • Humans
  • Kidney Failure, Chronic / epidemiology
  • Kidney Failure, Chronic / genetics*
  • Kidney Failure, Chronic / surgery*
  • Kidney Transplantation*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Reference Values
  • Restriction Mapping
  • Risk Factors
  • Tissue Donors / statistics & numerical data
  • Treatment Outcome