Abstract
Octapeptide R2 (KVLDGQDP), which has anti-transglutaminas (TGase) activity, decreases inflammation in allergic conjunctivitis model in guinea pigs. The authors examined the effect of R2 on lipopolysaccharide (LPS)-induced lung injury in BALB/c mice. R2 inhalation significantly decreased neutrophil count and cytokine mRNA expression in the lungs of LPS (25 mg/kg)-treated mice (P < .05). It also showed a tendency for decreased tumor necrosis factor (TNF)-alpha-immunoreactive protein in lung homogenates and significantly decreased TNF-alpha-immunoreactive protein in the serum of LPS-injected mice (P < .05). These results indicate that TGase may be a new therapeutic target in LPS-induced lung inflammation.
MeSH terms
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Administration, Inhalation
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Animals
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Enzyme Activation / drug effects
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Enzyme Inhibitors / pharmacology
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Interleukin-6 / blood
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Interleukin-6 / genetics
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Lipopolysaccharides / pharmacology*
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Male
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Mice
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Mice, Inbred BALB C
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Neutrophils / cytology
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Neutrophils / immunology
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Peptide Fragments / pharmacology*
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Pneumonia / chemically induced
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Pneumonia / drug therapy*
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Pneumonia / metabolism*
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RNA, Messenger / metabolism
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Transglutaminases / antagonists & inhibitors*
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Transglutaminases / metabolism
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Enzyme Inhibitors
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Interleukin-6
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Lipopolysaccharides
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Peptide Fragments
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Transglutaminases