Abstract
LEDGFp75 is a cellular protein which binds human immunodeficiency virus type 1 (HIV-1) integrase with high specificity and affinity but whose function in infection has not been defined. We infected LEDGFp75-deficient primary macrophages with wild-type HIV in order to assess potential infection phenotypes which would provide clues to LEDGFp75 function. Silencing of LEDGFp75 by 70 to 80% resulted in an average of 53% reduced infection of macrophages by HIV. Analysis of infection intermediates showed that integration, but not two-long-terminal-repeat (2LTR) circles or late cDNAs, was reduced up to 74% in LEDGFp75-deficient macrophages. Therefore, LEDGFp75 has a modest involvement in HIV-1 integration in macrophages.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
Adaptor Proteins, Signal Transducing / antagonists & inhibitors
-
Adaptor Proteins, Signal Transducing / genetics
-
Adaptor Proteins, Signal Transducing / metabolism*
-
Cells, Cultured
-
Gene Silencing*
-
HIV Infections / genetics
-
HIV Infections / metabolism*
-
HIV Long Terminal Repeat / genetics
-
HIV-1 / physiology*
-
Humans
-
Macrophages / metabolism*
-
Macrophages / virology
-
Protein Isoforms / antagonists & inhibitors
-
Protein Isoforms / genetics
-
Protein Isoforms / metabolism
-
Transcription Factors / antagonists & inhibitors
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
-
Virus Integration / physiology*
Substances
-
Adaptor Proteins, Signal Transducing
-
PSIP1 protein, human
-
Protein Isoforms
-
Transcription Factors