Abstract
HAb18G/CD147 has been identified as a factor that induces MMPs production. SiRNA targeted against HAb18G/CD147 was transfected into FHCC-98 cells (a HCC cell line) to knockdown its expression. The results showed that downregulating HAb18G/CD147 decreased ERK1/2, MMP-2 and FAK levels and inhibited cell motility and invasion, together with rearranged actin stress fiber formation, while had no effects on integrin alpha3beta1 expression. MEK1/2 inhibitor, U0126, inhibited MMP-2, FAK and actin expression in FHCC-98 cell line. The findings indicate that si-HAb18G inhibits gelatinase production, actin and FAK expression in FHCC-98 via an ERK1/2 signaling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism*
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Base Sequence
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Basigin
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Carcinoma, Hepatocellular / enzymology*
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Carcinoma, Hepatocellular / pathology
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Cell Line, Tumor
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Focal Adhesion Protein-Tyrosine Kinases / metabolism*
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Humans
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Liver Neoplasms / enzymology*
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Liver Neoplasms / pathology
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Matrix Metalloproteinase 2 / metabolism*
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Mitogen-Activated Protein Kinase 1 / metabolism*
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Mitogen-Activated Protein Kinase 3 / metabolism*
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RNA, Small Interfering*
Substances
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Actins
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BSG protein, human
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RNA, Small Interfering
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Basigin
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Focal Adhesion Protein-Tyrosine Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Matrix Metalloproteinase 2