Abstract
A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Heterophile / analysis
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Autoradiography
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Blotting, Western / methods*
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Chromogenic Compounds
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Densitometry
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Electrophoresis, Polyacrylamide Gel / methods*
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Gliadin / immunology
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HIV Antibodies / analysis
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Horseradish Peroxidase / analysis
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Humans
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Hydrogen Peroxide
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Immunoglobulin G / analysis
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Isoelectric Focusing / methods*
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Luminescent Measurements*
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Luminol*
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Mice
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Transglutaminases / analysis
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von Willebrand Factor / analysis
Substances
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Antibodies, Heterophile
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Chromogenic Compounds
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HIV Antibodies
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Immunoglobulin G
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von Willebrand Factor
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Luminol
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Gliadin
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Hydrogen Peroxide
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Horseradish Peroxidase
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Transglutaminases