Abstract
The genes coding for 4 aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(3'), ANT(4') and ANT(6) were determined in 44 Slovak clinical isolates of Enterococcus faecalis with high-level resistance to gentamicin (HLGR, collection 1) and 48 E. faecalis isolates with resistance to amikacin (AR, collection 2). The occurrence of spotted genes was (collection 1 vs. collection 2): aac(6)-aph(2") 81.8 vs. 8.3 %, ant(4') 52.3 vs. 81.3 %, aph(3') 50 vs. 56.3 % and ant(6) 6.8 vs. 4.2 %, the most frequent combinations of genes in the HLGR collection were aac(6')-aph(2") + ant(4') and aac(6')-aph(2") + aph(3). In contrast, the aph(3') + ant(4') gene profile was predominant in AR isolates. None of the isolates contained all four AGME genes simultaneously.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases / genetics
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Amikacin / pharmacology
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Aminoglycosides / pharmacology*
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Anti-Bacterial Agents / pharmacology
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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Drug Resistance, Multiple, Bacterial / genetics*
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Enterococcus faecalis / drug effects*
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Enterococcus faecalis / enzymology
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Enterococcus faecalis / genetics*
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Enterococcus faecalis / isolation & purification
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Gentamicins / pharmacology
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Gram-Positive Bacterial Infections / microbiology
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Humans
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Microbial Sensitivity Tests
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Nucleotidyltransferases / genetics*
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Polymerase Chain Reaction
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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DNA, Bacterial
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Gentamicins
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Amikacin
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6'-aminoglycoside acetyltransferase-2''-aminoglycoside phosphotransferase
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Acetyltransferases
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Phosphotransferases (Alcohol Group Acceptor)
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streptomycin 3''-kinase
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Nucleotidyltransferases