Comparing the whole-genome-shotgun and map-based sequences of the rice genome

Trends Plant Sci. 2006 Aug;11(8):387-91. doi: 10.1016/j.tplants.2006.06.005. Epub 2006 Jul 13.

Abstract

The rice genome has now been sequenced using whole-genome-shotgun and map-based methods. The relative merits of the two methods are the subject of debate, as they were in the human genome project. In this Opinion article, we will show that the serious discrepancies between the resultant sequences are mostly found in the large transposable elements such as copia and gypsy that populate the intergenic regions of plant genomes. Differences in published gene counts and polymorphism rates are similarly resolved by considering how transposable elements affect the sequence analysis.

Publication types

  • Comparative Study

MeSH terms

  • Chromosomes, Plant
  • Cost-Benefit Analysis
  • DNA, Plant
  • Genome, Human
  • Genome, Plant*
  • Humans
  • Oryza / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Sequence Analysis, DNA / economics
  • Sequence Analysis, DNA / methods*

Substances

  • DNA, Plant