Repetitive nucleotide sequencing of a dispensable DNA segment in a clonal population of African swine fever virus

Virus Res. 1991 Aug;20(3):265-72. doi: 10.1016/0168-1702(91)90080-f.

Abstract

Repetitive nucleotide sequencing of a dispensable genomic segment of a clonal population of African swine fever (ASF) virus has been carried out to estimate the mutant frequency to neutral alleles. Since no mutations have been detected in a total of 54026 nucleotides screened, the maximum mutant frequency is 5.5 x 10(-5) substitutions/nucleotide (95% confidence level). The result renders very unlikely the occurrence of hypermutational events during ASF virus DNA replication, at least within the selected DNA fragment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Swine Fever Virus / genetics*
  • Base Sequence
  • Blotting, Southern
  • DNA Probes
  • DNA, Viral / analysis*
  • Humans
  • Infant, Newborn
  • Molecular Sequence Data
  • Mutagenesis
  • Mutation
  • Open Reading Frames
  • Polymorphism, Restriction Fragment Length
  • Repetitive Sequences, Nucleic Acid*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • DNA Probes
  • DNA, Viral