PRINS as an efficient tool for aneuploidy assessment in human oocytes and preimplantation embryos

Franck Pellestor; Brigitte Andréo; Tal Anahory; Samir Hamamah

doi:10.1385/1-59745-068-5:151

PRINS as an efficient tool for aneuploidy assessment in human oocytes and preimplantation embryos

Methods Mol Biol. 2006:334:151-60. doi: 10.1385/1-59745-068-5:151.

Authors

Franck Pellestor¹, Brigitte Andréo, Tal Anahory, Samir Hamamah

Affiliation

¹ Institute of Human Genetics, CNRS UPR 1142, Montpellier, France.

PMID: 16861761
DOI: 10.1385/1-59745-068-5:151

Abstract

An ultrarapid three- and four-color primed in situ (PRINS) procedure has been developed for rapid chromosome identification and aneuploidy assessment on isolated cells. Based on the direct in situ mixing of fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue), this multicolor PRINS procedure is described on unfertilized human oocytes and isolated human blastomeres.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aneuploidy*
Blastocyst / metabolism*
Blastomeres / metabolism
Chromosomes, Human / genetics
Female
Humans
Oocytes / metabolism*
Primed In Situ Labeling / methods*