[Antibody detection in sera of patients with Schistosomiasis japonica by dot immunogold filtration assay]

Jing Xu; Zi-zhu Yan; Rui-jian Zhang; Ting Feng; Qiang Wang; Cui-zhen Qian; Xiao-hua Wu; Dan Zhu; Jia-gang Guo; Xiao-nong Zhou

[Antibody detection in sera of patients with Schistosomiasis japonica by dot immunogold filtration assay]

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Apr 30;24(2):146-7, 155.

[Article in Chinese]

Authors

Jing Xu¹, Zi-zhu Yan, Rui-jian Zhang, Ting Feng, Qiang Wang, Cui-zhen Qian, Xiao-hua Wu, Dan Zhu, Jia-gang Guo, Xiao-nong Zhou

Affiliation

¹ National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.

PMID: 16862917

Abstract

Serum antibody of schistosomiasis patients was detected by dot immunogold filtration method (DIGFA) in laboratory and field, and F-ELISA was used as control. The results showed that there was no significant difference between these two assays in sensitivity and specificity (P > 0.05), with a high coincidence. DIGFA is easy to operate and may deserve a wide application in the diagnosis of schistosomiasis.

Publication types

English Abstract

MeSH terms

Animals
Antibodies, Helminth / blood*
Antigens, Helminth / immunology
Enzyme-Linked Immunosorbent Assay / methods
Humans
Immunoblotting / methods
Reagent Kits, Diagnostic
Reproducibility of Results
Schistosoma japonicum / immunology*
Schistosomiasis japonica / blood*
Schistosomiasis japonica / diagnosis
Schistosomiasis japonica / parasitology
Sensitivity and Specificity

Substances

Antibodies, Helminth
Antigens, Helminth
Reagent Kits, Diagnostic