Mixed monolayer-protected gold nanoclusters as selective peptide extraction agents for MALDI-MS analysis

Benjamin N Y Vanderpuije; Gang Han; Vincent M Rotello; Richard W Vachet

doi:10.1021/ac0604181

Mixed monolayer-protected gold nanoclusters as selective peptide extraction agents for MALDI-MS analysis

Anal Chem. 2006 Aug 1;78(15):5491-6. doi: 10.1021/ac0604181.

Authors

Benjamin N Y Vanderpuije¹, Gang Han, Vincent M Rotello, Richard W Vachet

Affiliation

¹ Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.

PMID: 16878887
DOI: 10.1021/ac0604181

Abstract

Cationic and anionic nanoparticles selectively target peptides with low and high isoelectric points, respectively. Additionally, their high surface area-to-volume ratios make these nanoparticles (approximately 2-nm core diameter) very efficient extraction and concentration agents. Upon extraction, the peptide-bound nanoparticles can be analyzed by MALDI-MS to provide highly sensitive detection of the targeted peptides. We demonstrate that MALDI-MS can detect peptide concentrations as low as 500 pM from 250-microL solutions using these nanoparticle scaffolds as extraction and concentration agents.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Gold / chemistry*
Hydrogen-Ion Concentration
Membranes, Artificial*
Nanostructures / chemistry*
Peptides / analysis*
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Membranes, Artificial
Peptides
Gold