Expression of human immunodeficiency virus type 1 tat from a replication-deficient herpes simplex type 1 vector induces antigen-specific T cell responses

Vaccine. 2006 Nov 30;24(49-50):7148-58. doi: 10.1016/j.vaccine.2006.06.061. Epub 2006 Jul 12.

Abstract

Herpes simplex type-1 virus (HSV-1) based vectors have been widely used in different gene therapy approaches and also as experimental vaccines against HSV-1 infection. Recent advances in the HSV-1 technology do support the use of replication defective HSV-1 as vaccine vectors for delivery of foreign antigens. We have examined the ability of a recombinant replication-defective HSV-1 vector expressing the HIV-1 Tat protein to induce long-term Tat-specific immune responses in the Balb/c murine model. The results showed that vector administration by the subcutaneous route elicits anti-Tat specific T-cell mediated immune responses in mice characterized by the presence of the Tat-specific cytotoxic activity and production of high levels of IFN-gamma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / administration & dosage
  • AIDS Vaccines / immunology*
  • Administration, Intranasal
  • Animals
  • Blotting, Western
  • Cell Proliferation
  • Chlorocebus aethiops
  • Enzyme-Linked Immunosorbent Assay
  • Gene Products, tat / biosynthesis*
  • Gene Products, tat / immunology*
  • Genetic Vectors
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / immunology*
  • Immunization Schedule
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / genetics
  • Injections, Subcutaneous
  • Interferon-gamma / immunology
  • Mice
  • Mice, Inbred BALB C
  • Plasmids / genetics
  • Spleen / cytology
  • Spleen / immunology
  • T-Lymphocytes / immunology*
  • Vaccines, Synthetic / immunology
  • Vero Cells
  • Virus Replication / genetics*

Substances

  • AIDS Vaccines
  • Gene Products, tat
  • Immunoglobulin G
  • Vaccines, Synthetic
  • Interferon-gamma