Abstract
We developed dinoflagellate-specific 18S rRNA gene primers. PCR amplification using these oligonucleotides for a picoplanktonic DNA sample from Long Island Sound yielded 24 clones, and all but one of these clones were dinoflagellates primarily belonging to undescribed and Amoebophrya-like lineages. These results highlight the need for a systematic investigation of picodinoflagellate diversity in both coastal and oceanic ecosystems.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cloning, Molecular
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DNA Primers*
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DNA, Protozoan / analysis
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DNA, Ribosomal / analysis
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Dinoflagellida / classification
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Dinoflagellida / genetics*
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Dinoflagellida / isolation & purification*
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Genes, rRNA
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods*
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RNA, Ribosomal, 18S / genetics
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Seawater / parasitology*
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Sequence Analysis, DNA
Substances
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DNA Primers
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DNA, Protozoan
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DNA, Ribosomal
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RNA, Ribosomal, 18S
Associated data
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GENBANK/DQ386737
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GENBANK/DQ386738
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GENBANK/DQ386739
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GENBANK/DQ386740
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GENBANK/DQ386741
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GENBANK/DQ386742
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GENBANK/DQ386743
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GENBANK/DQ386744
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GENBANK/DQ386745
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GENBANK/DQ386746
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GENBANK/DQ386747
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GENBANK/DQ386748
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GENBANK/DQ386749
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GENBANK/DQ386750
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GENBANK/DQ386751
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GENBANK/DQ386752
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GENBANK/DQ386753
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GENBANK/DQ386754
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GENBANK/DQ386755
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GENBANK/DQ386756
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GENBANK/DQ386757
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GENBANK/DQ386758
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GENBANK/DQ386759
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GENBANK/DQ386760
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GENBANK/DQ388456
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GENBANK/DQ388457
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GENBANK/DQ388458
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GENBANK/DQ388459
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GENBANK/DQ388460
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GENBANK/DQ388461
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GENBANK/DQ388462
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GENBANK/DQ388463
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GENBANK/DQ388464
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GENBANK/DQ388465
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GENBANK/DQ388466