Comparison of real-time PCR assays for monitoring serum hepatitis B virus DNA levels during antiviral therapy

J Clin Microbiol. 2006 Aug;44(8):2983-7. doi: 10.1128/JCM.02544-05.

Abstract

The performance characteristics of the RealART and Molecular Beacons assays were compared with those of the Digene Hybrid Capture II assay (ultrasensitive). The results of the RealART and Digene Hybrid assays were related (r = 0.94; P < 0.001) and diverged by 2 orders of magnitude. The RealART assay can be used to effectively monitor serum hepatitis B virus DNA levels.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Viral / analysis*
  • Drug Monitoring / methods*
  • Hepatitis B / drug therapy*
  • Hepatitis B / virology
  • Hepatitis B virus / genetics
  • Hepatitis B virus / physiology*
  • Humans
  • Polymerase Chain Reaction*
  • Sensitivity and Specificity
  • Viral Load*

Substances

  • DNA, Viral