Abstract
A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Transport / genetics
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Blood / microbiology
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Body Fluids / microbiology
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Burkholderia pseudomallei / genetics*
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Burkholderia pseudomallei / isolation & purification
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Burkholderia pseudomallei / metabolism
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DNA, Bacterial / analysis*
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DNA, Bacterial / genetics
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Endemic Diseases
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Humans
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Melioidosis / diagnosis*
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Sepsis / microbiology