Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination

Mol Biochem Parasitol. 2006 Nov;150(1):118-21. doi: 10.1016/j.molbiopara.2006.06.014. Epub 2006 Jul 25.
No abstract available

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimalarials / pharmacology
  • Cytosine Deaminase / genetics*
  • Flucytosine / pharmacology
  • Gene Deletion*
  • Gene Targeting
  • Humans
  • Pentosyltransferases / genetics*
  • Plasmodium falciparum / drug effects
  • Plasmodium falciparum / genetics*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Recombination, Genetic*
  • Saccharomyces cerevisiae / enzymology
  • Selection, Genetic*
  • Tetrahydrofolate Dehydrogenase / genetics
  • Transfection
  • Triazines / pharmacology

Substances

  • Antimalarials
  • Recombinant Fusion Proteins
  • Triazines
  • BRL 6231
  • Flucytosine
  • Tetrahydrofolate Dehydrogenase
  • Pentosyltransferases
  • uracil phosphoribosyltransferase
  • Cytosine Deaminase