Abstract
We tested whether glutamate receptor ligands affect oxygen-glucose deprivation-evoked L-glutamate efflux from adult rat cerebrocortical prisms. The uncompetitive NMDA antagonist AR-R15896AR inhibited efflux (IC50 34 microM, 87% maximal inhibition). AMPA/kainate receptor blockade (NBQX, 100 microM) or Group II metabotropic glutamate receptor activation (DCG-IV, 10 microM) inhibited efflux (41%, 67% respectively) but Group I mGluR blockade (CPCCOEt/MPEP, 10 microM) was without effect. These data support a modulatory effect of glutamate receptors on L-glutamate efflux.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism*
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Dose-Response Relationship, Drug
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Excitatory Amino Acid Antagonists / pharmacology
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Female
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Glucose / deficiency
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Glutamic Acid / metabolism*
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Hypoxia / metabolism
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In Vitro Techniques
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Neural Inhibition / drug effects
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Neural Inhibition / physiology*
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Pyridines / pharmacology
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Quinoxalines / pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptors, Glutamate / physiology*
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Time Factors
Substances
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Excitatory Amino Acid Antagonists
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Pyridines
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Quinoxalines
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Receptors, Glutamate
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alpha-phenyl-2-pyridineethanamine dihydrochloride
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2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline
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Glutamic Acid
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Glucose